Arthur Hosie left this group in the spring of 2003. He is now Lecturer in Molecular Microbiology in the Department of Microbiology, GKT Dental Institute, King’s College London. He continues his research interests in the function and mechanism of action of ABC and other microbial transporters. More Information can be found here.
Before arriving in this group, Arthur had a diverse research history. After graduating from The University of Glasgow in 1991 (BSc (Hons) Microbiology), he moved to the Moredun Research Institute in Edinburgh, where he worked as a scientific officer studying the molecular biology of Mycobacteria associated with Johnes disease, especially M. avium silvaticum and M. avium paratuberculosis. In 1994, he returned to The University of Glasgow to undertake my PhD research under the supervision of Dr. Harry Birkbeck (and Dr. Susan Gallacher at the FRS Marine Laboratory in Aberdeen). The aim of this project was to identify genes in marine bacterial isolates involved in the biosynthesis of sodium channel blocking toxins (i.e. tetrodotoxin and saxitoxin derivatives involved in paralytic shellfish poisoning). On completion of his thesis in January 1998, Arthur took up a position in this group.
While a Post-Doctoral Research Fellow in this group, his primary role was to characterise bi-directional solute movement in ABC transporters. During the course of this work, he characterised a second general amino acid transporter of R. leguminosarum (rlBra) with similarity to the LIV-I transporters of E. coli and P. aeruginosa, members of the HAAT family of ABC transporters. Ongoing research has indicated that the two general amino acid permeases (i.e. Aap and rlBra) are essential for effective nitrogen fixation, data that have questioned the paradigm of symbiotic nitrogen fixation by R. leguminosarum. The completed genome sequences of a number of alpha-proteobacteria, including Sinorhizobium meliloti, and Mesorhizobium loti, have revealed a multiplicity of ABC transporters. A challenge for the future is to determine the role each of these transporters has in the physiology of Rhizobia.
Publications
E. M. Lodwig*, A. H. F. Hosie*, A. Bourdès, K. Findlay, D. Allaway, R. Karunakaran, J. A. Downie and P. S. Poole (2003) Amino-acid cycling drives nitrogen fixation in the legume-Rhizobium symbiosis. Nature 422 722-725.
*These authors contributed equally to this work
A. H. F. Hosie, D. Allaway, and P. S. Poole (2002) A monocarboxylate permease of Rhizobium leguminosarum is the first member of a new subfamily of transporters. Journal of Bacteriology 184 5436-5448.
A. H. F. Hosie, D. Allaway, H. A. Dunsby, C. S. Galloway, and P. S. Poole (2002) Rhizobium leguminosarum has a second general amino acid permease with unusually broad substrate specificity and high similarity to branched-chain amino acid transporters (Bra/LIV) of the ABC family. Journal of Bacteriology 184 4071-4080.
R. A. Carter, K. H. Yeoman, A. Klein, A. H. F. Hosie, G. Sawers, P. S. Poole and A. W. B. Johnston (2002) dpp genes of Rhizobium leguminosarum specify uptake of d-amino-levulinic acid. Molecular Plant-Microbe Interactions 15 69-74.
A. H. F. Hosie and P. S. Poole (2001) Bacterial ABC transporters of amino acids. Research in Microbiology 152 259-270.
A. H. F. Hosie, D. Allaway, M. A. Jones, D. L. Walshaw, A. W. B Johnston and P. S. Poole (2001) Solute-binding protein-dependent ABC transporters are responsible for solute efflux in addition to solute uptake. Molecular Microbiology 40 1449-1459.
A. H. F. Hosie, S. Gallacher and T. H. Birkbeck (1998) Transposon mutagenesis of marine bacteria which produce paralytic shellfish poisons. p. 279. In : Harmful Algae, eds. B. Reguera, J. Blanco, M. L. Fenrandez and T. Wyatt. Xunta de Galicia and Intergovernmental Oceanographic Commission of UNESCO.
N. F. Inglis, K. Stevenson, A. H. F. Hosie and J. M. Sharp (1994) Complete sequence of the gene encoding the bacterioferritin subunit of Mycobacterium avium subspecies silvaticum. Gene 150 205-206.
